Automated DNA sequencing methods involving polymerase chain reaction.
نویسندگان
چکیده
منابع مشابه
Automated DNA sequencing methods involving polymerase chain reaction.
Polymerase chain reaction (PCR) as a method for preparing DNA templates has been used for several DNA sequencing applications. An in situ procedure for directly sequencing PCR products by the dideoxy-termination method has been developed by using fluorophore-labeled sequencing primers. Completed sequence reactions were combined and loaded into a single electrophoretic lane of a fluorescence-bas...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1989
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/35.11.2196